Journal: bioRxiv
Article Title: Females are protected from semaglutide-induced muscle loss in ob/ob mice
doi: 10.64898/2026.03.03.709376
Figure Lengend Snippet: A: A: Immunohistochemical characterization of tibialis anterior (TA) muscle fiber types. TA muscle cross-sections were stained to identify individual fiber types: Type IIb fibers appear red, Type IIa fibers appear blue, and unstained fibers (appearing black) correspond to Type IIx. Wheat germ agglutinin (WGA) labeling outlines muscle fiber borders in green, enabling clear visualization of fiber morphology and distribution. B & D: Showing muscle % fibers distribution per cross sectional area in vehicle or semaglutide treated male and female mice. C & E: Showing muscle % fiber composition in vehicle or semaglutide treated male and female mice. Data is represented as mean ± SEM. n=3/group for male and n=4/group for female.
Article Snippet: Following the 4-week acclimation period, mice were randomly divided into two experimental groups where one group received daily subcutaneous injections of 3 nmol/kg body mass semaglutide (Novo Nordisk), and another group received daily subcutaneous injections of an equivalent volume of vehicle (phosphate-buffered saline, PBS).
Techniques: Immunohistochemical staining, Staining, Labeling